The B.X. Cancer Virus
BX Cancer Virus


Dr. Rife Talks About His Work On The Cancer Viruses Of BX and BY


Dr. Rife's talk about the BX and BY Cancer Virus was recorded by John Crane in the 1950's.
Transcribed from the original tape recording.
Copyright 2006

Rife: As far back as 1920 I conceived the idea and the possibility of when the causative agent of malignancy, so called cancer, would be discovered and found and proven that it would be caused by a microorganism. Of course, reception that I received that far back from the medical profession and scientists was nil but I kept at the work and I succeeded in eventually isolating a virus. 

First, I began sectioning tissues of every known type of malignancy. I sectioned over 20,000 of those, cut them down with a special micatone to very thin. Some of them were only a micron in thickness.  I studied those under the microscope and I eventually built my first high-powered microscope for the purpose of analyzing and checking those sections. The only results that I obtained over all those years was I succeeded in developing a very excellent technique of tissue preparation. But I never found an organism that I could say was the causative agent of malignancy. 

I went through with this and then I developed other instruments of greater amplitude of magnification than the old original number one which was very lacking in resolution beyond about 8 or 9 thousand times. It was a lens microscope and all of the air was excluded from the body and replaced with glycerin. The lenses were homogeneous with glycerin all the way through the whole thing and the result was that in allowing the rays to separate and not cross in the interference band of reflection such as they do in the ordinary standard tube of a regular research microscope, we held them apart. We separated them and then brought them back together and picked them up again at a needle point. But as I say, the resolution of this instrument beyond about anywhere from 9 to 10,000 times dropped off decidedly rapidly. We could go up on some occasions on some preparations up to 17,000 times but we didn’t consider the resolution anything out of the ordinary beyond about 8 or 9 or 10,000 times depending entirely upon the specimen we were examining. With the results that those tissues gained us absolutely nothing. And as I say, I built other microscopes beyond this one.  But that instrument we used up until 1931. 

With that instrument, Arthur Kendall and I, working jointly in the Pasadena General Hospital, succeeded in isolating what we classified the first filterable form of bacteria ever seen. It was isolated from the bacillus typhosus from cultures that Dr. Kendall brought from his laboratory in Northwestern University in Chicago. And we succeeded definitely in isolating a filterable form of that bacteria. Now these filterable forms are very minute in size. The smallest of all is the one from the BX which we isolated from cancer. We have never made a positive claim that that is a causative agent of cancer but that is the smallest and is less than a 1/20th of a micron in dimension and highly motile. 

We succeeded in isolating this organism, this BX, using a Kendall media. It’s known as Kendall media. It’s made of a desiccated pig intestine dehydrated down where it requires about 10 gallons of the original raw gut. When it’s desiccated and run through the extractor and dehydrated to make about 1 pound of the original material. Now that is placed in a tube. We use about 4 grams and then we use 10 to 20 CCs of a tyrode solution. It’s a chemical series of salts.  Now that is what Kendall used for the isolation or bringing out this bacillus typhosus in the filterable state. Which is published in the Californian Western Medicine in 1931. Our joint report on that. 

Now these organisms again before we go farther, they cannot be stained with the aniline or acid dye stains that’s used in the ordinary technique and method of staining tissue sections or even bacteria. Now those organisms are so minute and so delicate that they have to be stained with a frequency of light. Now after we built our first high-powered microscope, we had to build an illuminant unit to be able to see these different types of organisms and different types of tissue also. So, we had to build this particular unit. We built a unit which I have a patent on, special lamp that will produce 2,000 candle power in a needle-pointed beam where it comes through the condenser. But between this source of illumination and this so-called sub stage condenser, we have a pair, on the universal microscope we have four, but we use a pair of rotating wedge shaped quartz prisms. They are circular and they’re wedge-shaped. And by rotating those we bend a beam of light.  That’s what we termed the neutral or core beam of the illuminant unit. At different angles of incidence so it extracts the object under examination at different angles of refraction. 

There we see these particles at a different refractive index and every one of them is entirely different. For instance, we find that the bacillus typhosus in its filterable state is a beautiful turquoise blue body. We see them swimming through the field.  They are highly motile and incidentally, if the organism, the bacteria, is a motile bacteria, in most instances the virus will be motile or highly motile and vice a versa. So we see these beautiful turquoise blue bodies swimming through the field.  With that we can differentiate absolute and positively which we have done. We can analyze the blood of a typhoid suspect, the filtered blood serum or the urine. As many as ten and 15 days beyond or before a Weetall retraction will show that that patient has typhoid.  Which of course speeds up the diagnostic value of the work. 

So, we place a colon bacillus under this monochromatic light and there we have a reddish brown organism that is highly motile also. We place the filterable form of the bacillus of tuberculosis there. We have a shade green and so on. We place the filterable form or the BX that’s isolated from our carcinoma and we have a purplish red one.  And so on.  We have from the filterable form of poliomyelitis we have a coral pink. The value of this particular method, the principle, is as stated before, highly valuable in the diagnosis of a case. As we go on with this, it’s so important to have a positive diagnosis that is not interfered with by some laboratory faulty technique, staining methods and whatnot. 

Now as we stated before we’ll go back to the point where we contemplated isolating an organism from malignant tissue.  The thing is that I positively believed that when the causative agent was found it would be caused to be found by a microorganism. And not unlikely an organism that we have with us at all times that is changed by the metabolic shift of the human body itself. That we have proven. I also stated that in one stage it would be not unlikely that it would be found to be in the blood stream. That was also proven. So as we isolate this organism we have what we call the BX. It is a very complicated method and technique. The first material that we isolated was from an un-ulcerated breast mass that I received from the Paradise Valley Hospital in National City. We used and chose an un-ulcerated breast mass because we have less chance of outside contamination.   

I took those blocks about 4 millimeters square and I placed them in my K-media.  I incubated them. I had no results.  I changed them at different temperatures. I had no results. I was running at that time possibly 400 transfers a day and determining the effect that the argon, neon and krypton gas using as a bombardment in a loop would have upon the growth of pathogenic bacteria. We wanted to determine if it would stimulate or retard the growth. I was running those things through as I say four and five hundred transfers a day. And there was a vacant tube, a loop, that was standing and this one tube that I had that had this malignant tissue in, was standing on the bench along there and it might just as well had a red flag on it. 

So I picked this thing up and I dropped it in this argon loop. I left if for 24 hours until I was making my transfers the next day and I looked at it.  I saw that it had a cloudiness in it which it did not have before. Well, I examined it immediately under the microscope and I found nothing and I chemically analyzed it and I found out that it had been ionized by this bombardment of this 5000 volts in this argon loop. So there is only one counteraction of ionization. That’s oxidation. So I put it in a 2-inch water bath. A 2-inch vacuum and left it in the incubator for 24 hours. And I brought it out and it had changed again. And I immediately put it under the microscope and began rotating my prisms and I finally found it alive and teeming with one of the smallest of any of the filterable forms that we had yet seen in a purplish-red refraction under the monochromatic light. They were less than a 20th of a micron in dimensions. 

Well, that didn’t mean a great deal in the way that I carry on my work because it had been standing around over a period of time and anything might have happened to it. So we started in and run the process through again with the same identical technique with identically the same results. We ran those through many, many times.  I think 104 to be exact.  And carrying a control with each one at each test. The controls always remained negative and then we always had positive results of obtaining our BX. Then we began our animal experimentation with this. We chose the female albino rat.  Now the method of inoculation which we used is a technique in itself. The animal is kept under quarantine for no less than 10 days, sometimes 12. And its blood checks are made and stool analysis and so on. We want to be absolutely sure that that animal is not susceptible or has any other type of disease. So the animal is shaved and it’s kept under a partial anesthesia because the shock of the hypodermic needle sometimes has an effect upon the metabolism of those small animals.  We use a long, sterile hypodermic needle that’s filled with petroleum sterile jelly. And the inoculation is made no less than 25 or 30 millimeters under the epidermal layer up into the mammary gland. The result is after about 4 or 5 days we have a lesion that breaks out in the thyroid area. That we have never been able to determine the actual cause of that lesion that breaks out but it invariably does. But it heals in a few days and the tumor starts.  And many of those tumors if they are allowed to grow will carry a more gram weight than the animal itself. They grow very rapidly on account of the exceedingly high metabolism of the albino rat. 

We carried that through over a period of many, many experimental animals. And the result was always the same. Now then we come into the field of microbiology, and biochemistry, we find that these organisms can be readily changed to other organisms. We have classified in a whole entire category of so-called pathogenic bacteria 10 individual groups. And we find almost definitely that any organism within that group can be changed to any other organism within its own individual group by the media upon which it is grown. As an example, we can take a pure culture of colon bacillus. I mean an absolute pure culture that’s laboratory tested with no contamination or anything of that sort. And we can change and alter its media 2 parts per million per volume and in 36 hours we have a typhoid bacillus with every known laboratory check and test even to the Weetall retraction. So those organisms can be readily changed. 

But now we’ll come to the BX again. The BX will pass through the porosity of the W Berkefeld filter which is known as triple ought china. It’s what the Dresden china is built of. Of course, they are unglazed. But it’s a very fine porosity. Now we alter the media slightly of that organism in the tube and we have another purplish red organism that we call the BY. Now this organism is considerably larger than the BX. It will no longer pass the porosity of the W Berkefeld, we have to use what’s classified as an N for this organism to pass. But it is still in the filterable stage. So, as this goes on through these different stages, it can be readily changed back to a BX and produce the tumor.

Now then we alter the media again. We use a blood serum on this particular type of media instead of the K. And we have another organism that we find there from this identical BY or BX either. A monoccocoid organism. Now these organisms with a proper staining method can be seen with a good research microscope.  And we find them in over 90% of the blood in carcinomatis individuals. In the monocyte only. And the monocytes are the only of the white cells which have no digestive power or what we call phagocytosis. Now we alter the media once again and we place it this time on a hard base media built of agar and asparagus, ground green asparagus. Tomato will do also, but the asparagus is more potent. There we have a fungoid organism with all the branching forms.  It comes in and we have classified it as cryptomyces pleomorphia.  It’s a pleomorphic fungi. Now it comes in through the hyphe and on through up into the spores and the ascospore and all its branching forms. And as the ascospore when they shed or come out from the buttons of the spores themselves are for a very short time highly motile. That is, we presume they are. It may be a Brownian reaction as far as we are concerned on that because it isn’t a true motility they just have a movement. 

Now, we allow that particular culture to stand. We can throw that back at any moment into a BX in 36 or 48 hours and produce a BX. But we allow that culture to stand. It’s what we term a dormant stock culture.  For the period of metastases. Now it is very seldom that we have a true case of metastases from a removal of the initial mass of one portion of the anatomy so where it’ll reoccur and shows up in another. Less than a year. We allow that particular culture to stand for a period of one year as a dormant stock culture. We plant it back on its own asparagus based media. We allow it to incubate and grow. We do not have a cryptomyces pleomorphia.  We do not have a monococcoid organism which we find in the blood stream of carcinomatis patients. We do not have a BY or we do not have a BX. But we have, from an organism that was isolated initially from an un-ulcerated human breast mass, we have a colon bacillus. It will pass every known reaction of laboratory technique. It is important to record these things, which of course we have. But the thing of it is, it is important that the metabolism of the individual can readily change an un-pathogenic organism into a pathogenic organism bug that will absolutely produce A to Z. 

There has been much controversy as to the different classifications and qualifications of malignancy. Many people believe that the sarcoma will turn into a carcinoma. We have found in some experimental animals that that is true. But nothing that we can place as absolute.  Now the different types we have done very little work on the sarcoma because we do not associate that as a malignant tumor. In comparison with the work we have done with the true carcinoma. And we have a type that is a bone tumor called shrouganoma. Now that is a true malignancy but it is a malignancy of the bone joints and the bone marrow. That affects the type of your white blood cells greatly.  Many of our types of anemia will come from that and leukemia and so on. But we cannot absolutely say that that is a malignant tumor because we have not been able to isolate a BX from it. 

We must be able, with the organism that we isolate from a certain culture, to be able to place that into the experimental animal and produce the identical tumor which, on removal of that tumor surgically from the experimental animal, recover again that identical organism. Which we have done in over 700 cases of experimental animals. As we place the BX in the tissues of the animal we allow that tumor to grow and it’s removed surgically. The animal is very seldom ever harmed outside of the removal of the tumor and we section that tumor and we also study its pathology and we also check and produce a BX. That is what’s known as Coke’s postulate. Where we inoculate the animal. We recover from the animal the organism that we have placed in it in its true identical form and it can go on through as many transplants as desired. But when we can take an organism that is isolated from a human being and plant it into an experimental and recover all the true pathology of neoplastic tissue from that tumor in the animal and again recover from that tumor the BX that can be placed into another animal and produce the tumor why we consider the cycle complete of our series. 

For many years, laboratory workers and technicians have been producing tumors in animals, especially rats and mice. But they are not inoculating the virus, they are inoculating the cells from the ground up tumor. Which does not signify anything in our work at all. Because that’s been done over a period of years. It’s interesting but it does not show us anything. What we want to know is the end result of the accomplishment of the placing of this organism in the tissue of the animal. 

Many people have attempted to use guinea pigs for work on malignancy. Well, we have had no luck with it whatsoever.  Guinea pigs are very susceptible to your tuberculosis and different diseases of that sort but we have never been able to produce a true tumor in a guinea pig. They have sometimes a spontaneous tumor that resembles a malignant growth but it will not show in true pathology the end results. So that is what we wish the same as we isolate the filterable form of the poliomyelitis. As I say, we have a coral pink object there which is non-motile. 

Now, I worked a long time and Rosenow and I worked together.  Dr. E. C. Rosenow the head of the research department of bacteriology in Mayo’s. He worked for many years of the isolation of this organism, and he worked with a brain emulsion. I found the organism in the spinal fluid. The true virus. We attempted to use different types of animals.  Chimpanzees, monkeys and so on and we found the only true susceptible experimental animal to the adaption of the virus of polio was the albino rabbit. With those results we produced all the true paralysis in the animals. It had all the symptoms and the whole thing. The organism that’s isolated, Rosenow still calls it the virus of the streptococcus of poliomyelitis  which it may be that. I will not contradict because he has worked for many years on the streptococcus organism that he has isolated from the brain emulsion of patients susceptible to polio.  But we do not find that the other animals will take it. Only the albino rabbit with all the true symptoms. And those organisms can be changed around as we stated before by the metabolism. 

There’s been much controversy about the different types and the association of different types of tumors malignant and what we classify as nonmalignant tumors such as the sarcoma and so on. We have done little or no work on the true sarcoma as to its relative association with the true malignant tumor. We have worked some with the sarcoma of the Bard Rock Chicken which is known as a rous-sarcoma. It is an interesting organism. It is not a truly a filterable form. It can be seen readily with a research microscope on sections of the tumor. But it is interesting in one particular phase that it is a transmissible disease. It can be transmitted from one chicken to another and incidentally, the only fowl known to science that is susceptible to this rous-sarcoma is the bard rock chicken. They have it and it gets in the flock and they all have it.  Experimental work that we did with that was obtained most of our tumors from the zoo hospital in San Diego at one time back in ‘round ‘28 or ’29 somewhere in there. They gave me some of those tumors and I experimented with them some. We never attempted to transmit them back by inoculation or transplanting of any other way into the chickens. But we did do some work on them and we can see by the section of it on a real high power of a good research microscope with a proper staining method. 

Incidentally, the staining method that we use on that is very similar to the staining that we use as a flagellate stain. Now I developed a number of years ago a stain for flagellated organisms such as the b.coli and the typhoid and many others that is the most outstanding of any of the stains that I have tried to use and I have tested and checked through all the flagellate stains that was ever built. And this particular stain is made from an ink that we produce from alfalfa hay. The material is taken in a very small test tube and agitated slowly with one or two CCs of mercury and we presume that this mercury has the same effect as the tinning of a wire when you wish to solder it. And you take this ink that’s distilled or extracted from the alfalfa hay and put in there and move it back and forth very slowly in an agitation. Not shaking it or anything of that sort, and there you will put your material out.  Of course, they are dead. They are no longer motile because the stain will kill them. And there we see the beautiful flagellates. 

We developed this particular stain for this reason. Because in many cases of typhoid, we have a high contamination of colon bacillus. And by staining the mass material we can take and under the microscope we can determine by our counting chamber the number of coli and the number of typhoid that we have in a certain series of squares in the counting chamber. Now it’s just the same as the staining of the organisms. It’s a delicate technique that we stain them with the monochromatic light. And certain blood organisms we can stain with a monochromatic light. Even a larger one that cannot be stained with any acid or aniline dye stains. So as we go through with this thing on a long period of research, we find that this staining method in certain phases of this is of vital importance. Now as I stated before, we cannot see or stain the virus because the particles of the virus are much more minute than the chemical granules of the stain that we attempt to stain them with. So it’s the old saying, the mouse he cannot swallow the elephant. 

So as we go on with these different types of tumors, you take for instance the epithelioma, which is a true type of skin cancer.  We have isolated from epithelioma not numerous times, but a few times, our identical BX. We have isolated it from the tumors of the pyloric orifice of the stomach and so on. We get the identical BX from the true cancer anything that we can get true pathology from on our sectioning and staining method under the regular microscope. We can isolate from that particular organism a BX almost 100%. As we examine the blood of a carcinomatis individual, we find little or no real action. We know that in the monocytes of the white cells of the blood, we find these small monococcoid granular organisms, but we have as yet to find any effect on the red cells that malignancy has. The white cells will come to any combat to any infected area. And they will attempt to devour the virus which they do to an extent. But the growth of malignancy is so much more rapid than they can compete with that it’s just almost difficult. And the monocytes as we stated before are the only ones.

We’ve run our blood counts through our hemocytometer, through our actual blood counts, of the cells before and after the treatment of these cases.  Or before and after the removal of the tumor. And, we find a change in the blood count. The normal count of cells as any laboratory technician knows for the average individual is around about 5 million per cubic millimeter. Well, that will rise or lower and in a case of a severe case of malignancy of the liver or the lung, the count will drop down to 3 to 3 ½ million on the individual and a female much more. The white count will generally rise.  But we count those in a hemocytometer. We use an ordinary staining system and we use a pipette that is calibrated for red cells and white cells on a certain amount of dilution and it is a waste of time, of course, to explain this method and the principle of this thing because any laboratory technician does it hundreds of times a day. But as we run our counts, we find that it is a general lowering of the count in the case of malignancy over normal and generally a rise in the white count of the blood. 

The lymphatic system can absorb a certain amount of toxins that are thrown off and beyond that why then it is produced and it comes back again into the blood stream. But as far as any deterioration that we have ever seen they claim, in some cases a myelogeneous leukemia. They call that cancer of the blood. Well, possibly so, but it’s a deterioration of the blood especially the white cells the count rises up into triple and quadruple their normal amount and the red count generally deteriorates down to as low as 2 million. But it does not signify anything because we find in that particular type of malignancy of the blood as it’s termed, myelogeneous leukemia, we find the bas [Basophil] cells, the eosinophil cells the polyomorpheneucials and so on their morphology has changed greatly. But it is only the disease that has changed the blood and not the blood that has changed the disease. 

We consider as this particular stage as we call the monococcoid form of this BX enters into the blood stream through the lymphatic system it is carried through basically by the hemodin and the blood serum which we classify as hemoglobin of course. There in reality is no such a thing as a red cell or a white cell. They are merely classified as that particular type to differentiate. So, in the field of hematology, we go through and we study this blood in all stages and forms. And I stated before, we find that the only type we can actually distinguish that is associated with malignancy is found in the monocytes of the blood. And it is the monocytes as stated before, are the only of the white cells which have no phagocytosis. 

There is much controversy as to the P.H. of the individual and also of the test tube in the laboratory. Now we have found, experimenting with these different types of so-called malignant bacteria, that some are susceptible to an acid and some to different types of base. They’re positive or negative, alkaline or acid. Some bacteria are susceptible to the acid type and some to the reverse. But, we believe that an individual that has an absolute neutral metabolism is susceptible to no disease. Because we can take and we have repeated this experiment not once but hundreds of times in the laboratory with very careful and positive techniques, that we place three test tubes, one acid, one alkaline, the center tube is a neutral ph.  There is not an organism in the whole category of so-called pathogens will grow on that neutral P.H. And as I say, it depends entirely upon the acidity or alkalinity of the organism of which we wish to grow. 

To make a pure culture of these organisms especially the virus, it is a very technical long drawn-out process. Some of them are so-called anaerobic and some of them are oxiroebic. Some of them require oxygen such as the BX requires oxygen in one stage of it form to grow.  You take the poliomyelitis. It is strictly anaerobic; it has to be developed in a media that is absolutely free from air. The best media that we have found so far is what is known as a chicken fusion broth. We take the chicks just un-hatched, use the brain and make an emulsion and we grow the polio on that because it is associated and has almost the same affinity as the spinal fluid of the human being. With the results of that. As stated before, we believe that if the metabolism of the human body is absolutely normal and a neutral P.H. it is susceptible to no disease. 

Crane: “Uh, Doc, will you mention the polarity that you encountered with these viruses under the microscope?”

Well some of these particular organisms, not only the virus, but many of the pathogenic organisms have an individual polarity. Now we have tested those out and we find that the positive organism will not associate with the negative organism. We show that where we use a one-millionth of a volt under the slide in our hanging drop and some of the organisms will come to one side and some the other. Now for instance, in a case where we get back to the BX. That is a bipolar organism. It is susceptible, partial to, one pole and some to the other. We throw the negative pole and many of the motile organisms will come to that pole. We throw the positive pole and the rest of them that are motile and swimming will go to the other pole. But we go in with the micromanipulator, with our micropipette and we draw off from this drop the negative side. We transmit that into the animal and we have no malignant growth. We draw off the positive side. We put that into the experimental animal and we have no growth. But if we take the material from both the positive and negative pole, there we produce the true malignancy of neoplastic tissue. 

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